Composite

Part:BBa_K3002226:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + ARS_Mut-PETase_SP20His + ccA_MHETase_ SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal NheI site found at 2665
    Illegal NheI site found at 5618
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
    Illegal NotI site found at 7031
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal BglII site found at 7941
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 4955
    Illegal NgoMIV site found at 6629
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002121 (ARS_Mutant PETase_SP20HA) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).

References